Tracing how Kosovo Albanians and their international supporters staged Kosovo's diplomatic performance, and how the Yugoslav/Serbian delegation tried to undermine it, I demonstrate that diplomatic representation can indeed generate agency. Equipped with this conceptual toolkit, I subsequently turn to the story of Kosovo's representation at the Rambouillet conference held in 1999. Taking cue from Bruno Latour's and Lisa Disch's writings on political representation, I then suggest an alternative understanding of diplomacy that takes its performative character seriously. Therefore, to clarify what is at stake in the theoretical debate, I begin with a systematic discussion of how IR has conceived of diplomatic representation. role of diplomacy in the constitution of agency. Thus far, however, the academic discipline of International Relations (IR) has largely failed to acknowledge the. It demonstrates that, in the world of diplomacy, entities like 'Kosovo' can act only when they are carefully staged. This article traces how Kosovo came to agree to the Rambouillet accords, with the aim of exploring the nexus between diplomatic representation and international agency. The emotional state and shopping value created by the shopping environment were found to influence enjoyment of shopping, willingness to talk to salespeople, revisit intentions, and tendency to spend more money and time than originally planned. This research explored the relationships between the shopping environment and tourists’ emotions, shopping values and approach behaviours. The environment of shopping locations at destination must be pleasing and induce approach behaviours for the retail sector to be successful. If macroenvironment is not attractive and inviting, the rest of the in-store atmosphere may not matter. Exterior environment in shopping districts at destinations deserves attention in that macroclimate is the first set of cues. In this context, the shopping environment itself may become part of the tourist's experience influencing subsequent shopping behaviours and evaluations. Research and anecdotal evidence suggests that satisfaction from shopping is not necessarily derived from acquiring goods.
These two complimentary techniques, both involving use of the long PCR paired with restriction digestion, have potential application in any laboratory in which PCR is performed. Restriction digestion performed prior to long PCR amplification can be used to selectively suppress the amplification of members of families of closely related DNA sequences, thereby making it possible to selectively amplify one of a group of highly homologous sequences. The same primers used to perform the long PCR amplification can be used as probes to perform restriction mapping of the DNA fragment amplified. This article describes two novel applications of the long PCR technique, one which simplifies restriction mapping and another which enhances amplification specificity and yield. Applications for PCR have been extended significantly by the development of "long" PCR, a technique that makes it possible to amplify DNA fragments up to 40 kb in length. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.The polymerase chain reaction (PCR) is the most widely used technique for the study of DNA. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results.Ĭomprehensive and highly practical, E. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. coli, constitutes one of the fundamental methodologies of molecular biotechnology. Manipulation of recombinant DNA, which is almost exclusively performed using the host E.
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